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In order to study the molecular evolution of mammal QRFPR, we searched for the presence of natural selection on the qrfpr genes using a bioinformatic approach. The structure, tissuespecific expression and biochemical activity of the 26RFa/QRFP–QRFPR system are conserved across the Chordata phylum, from fish to mammals. 26RFa/QRFP is the high-affinity endogenous ligand for QRFPR and in fish it has been suggested as an orexigenic action of 26RFa/QRFP. These paralogous genes emerged along with speciesspecific gene or genome duplications that occurred during vertebrate evolution. Synteny analysis revealed the existence of paralogous genes of QRFPR in mouse, zebrafish and coelacanth.
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